Structure and activity of the RNA-targeting Type III-B CRISPR-Cas complex of Thermus thermophilus.

  title={Structure and activity of the RNA-targeting Type III-B CRISPR-Cas complex of Thermus thermophilus.},
  author={Raymond H J Staals and Yoshihiro Agari and Saori Maki-Yonekura and Yifan Zhu and David W. Taylor and Esther van Duijn and Arjan Barendregt and Marnix C. Vlot and Jasper J. Koehorst and Keiko Sakamoto and Akiko Masuda and Naoshi Dohmae and Peter J. Schaap and Jennifer A. Doudna and Albert J. R. Heck and Koji Yonekura and John van der Oost and Akeo Shinkai},
  journal={Molecular cell},
  volume={52 1},
The CRISPR-Cas system is a prokaryotic host defense system against genetic elements. The Type III-B CRISPR-Cas system of the bacterium Thermus thermophilus, the TtCmr complex, is composed of six different protein subunits (Cmr1-6) and one crRNA with a stoichiometry of Cmr112131445361:crRNA1. The TtCmr complex copurifies with crRNA species of 40 and 46 nt, originating from a distinct subset of CRISPR loci and spacers. The TtCmr complex cleaves the target RNA at multiple sites with 6 nt intervals… CONTINUE READING
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