Structure and activity of glycosylated human interferon-gamma.

  title={Structure and activity of glycosylated human interferon-gamma.},
  author={Tsutomu Arakawa and Y. R. Hsu and D N Chang and Nowell Stebbing and Bruce W Altrock},
  journal={Journal of interferon research},
  volume={6 6},
Structural properties and activity of recombinant human interferon-gamma (IFN-gamma) purified from Chinese hamster ovary (CHO) cells or a natural source were determined and compared with those of Escherichia coli-derived IFN-gamma. One preparation of CHO-derived IFN-gamma showed three bands, with the middle band being a doublet, in a SDS-polyacrylamide gel. The two higher-molecular-weight bands were shown to be glycosylated. Western blot analysis indicated that the three bands are IFN-gamma and… CONTINUE READING

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