Structure and Sequence of the Human Fast Skeletal Troponin T (TNNT3) Gene: Insight Into the Evolution of the Gene and the Origin of the Developmentally Regulated Isoforms

@article{Stefancsik2003StructureAS,
  title={Structure and Sequence of the Human Fast Skeletal Troponin T (TNNT3) Gene: Insight Into the Evolution of the Gene and the Origin of the Developmentally Regulated Isoforms},
  author={Raymund Stefancsik and Jeffrey D. Randall and C Mao and Satyapriya Sarkar},
  journal={Comparative and Functional Genomics},
  year={2003},
  volume={4},
  pages={609 - 625}
}
We describe the cloning, sequencing and structure of the human fast skeletal troponin T (TNNT3) gene located on chromosome 11p15.5. The single-copy gene encodes 19 exons and 18 introns. Eleven of these exons, 1–3, 9–15 and 18, are constitutively spliced, whereas exons 4–8 are alternatively spliced. The gene contains an additional subset of developmentally regulated and alternatively spliced exons, including a foetal exon located between exon 8 and 9 and exon 16 or α (adult) and 17 or β (foetal… 

Figures and Tables from this paper

Polymorphism in troponin T gene (TNNT3) and its effect on gene expression level and production traits in pigs of selected breeds.
TLDR
TNNT3 polymorphism did not reveal any relation with the shank weigh without skin and backfat, loin eye,Meat content of primary cuts, meat content of carcass and meat weight in primary cuts; however, a relation was observed between the polymorphism of TNNT2 gene and the meat quality expressed by post-slaughter pH45 and pH24.
Identification of a Region of Fast Skeletal Troponin T Required for Stabilization of the Coiled-coil Formation with Troponin I*
TLDR
Surprisingly it was found that the HR regions alone of the fast skeletal TnT and TnI, as defined earlier, were insufficient to form a coiled-coil, and it was shown that an additional 14 amino acid residues N-terminal to the conserved HR region (TnT residues 165-178) are essential for the stable coiled -coil formation.
Expression of contractile protein-encoding genes TPM2 and TNNT3 during ontogenesis in pigs raised in Poland*
TLDR
Results suggest that an unknown polymorphism, probably located in regulatory part of the gene has an effect on the level of TNNT3 expression, which is a component of thin filaments in the developing muscle of pigs.
Effects of age and hindlimb immobilization and remobilization on fast troponin T precursor mRNA alternative splicing in rat gastrocnemius muscle.
TLDR
The results suggest that Tnnt3 pre-mRNA alternative splicing is modulated rapidly (i.e., within days) in response to changes in the load placed on the muscle.
Influence of ageing and essential amino acids on quantitative patterns of troponin T alternative splicing in human skeletal muscle.
TLDR
It is concluded that essential amino acid supplementation after resistance exercise may provide a means to reduce impairments in skeletal muscle quality during ageing in humans.
Maternal methionine supplementation during gestation alters alternative splicing and DNA methylation in bovine skeletal muscle
TLDR
Evidence is provided that a prenatal diet rich in methyl donors can significantly alter the offspring transcriptome, including changes in isoform expression and exon usage, and some of these changes are mediated by changes in DNA methylation.
Body weight-dependent troponin T alternative splicing is evolutionarily conserved from insects to mammals and is partially impaired in skeletal muscle of obese rats
TLDR
Weight-dependent quantitative variation in Tnnt3 alternative splicing appears to be an evolutionarily conserved feature of skeletal muscle and provides a quantitative molecular marker to track how an animal perceives and responds to body weight.
Alternative splicing regulates the physiological adaptation of the mouse hind limb postural and phasic muscles to microgravity
TLDR
It is proposed that substantial remodeling of pre-mRNA by AS is a major component of transcriptomic adaptation of skeletal muscle to microgravity and could be targeted by small molecule splicing regulator therapies to address microgravity-induced changes in muscle during spaceflight.
...
...

References

SHOWING 1-10 OF 65 REFERENCES
Origin of fetal troponin T: developmentally regulated splicing of a new exon in the fast troponin T gene.
TLDR
F fetal TnTs are generated from the fast TnT gene by the inclusion of a new, alternatively spliced exon, which is designated f (for fetal), whose developmentally regulated splicing appears to be a common feature of mammalian skeletal muscle development.
Isolation and characterization of human fast skeletal beta troponin T cDNA: comparative sequence analysis of isoforms and insight into the evolution of members of a multigene family.
TLDR
Comparative sequence analysis reveals that the human beta TnTf shares a high level of sequence similarity in the coding region with other vertebrate TNTf and considerably reduced similarity with slow skeletal and cardiac TnS cDNAs.
Identification of a fetal exon in the human fast Troponin T gene
Assignment of the human fast skeletal troponin T gene (TNNT3) to chromosome 11p15.5: evidence for the presence of 11pter in a monochromosome 9 somatic cell hybrid in NIGMS mapping panel 2.
TLDR
The study indicates the presence of an 11pter region in the NIGMS cell hybrid GM10611, which has previously been reported to contain only human chromosome 9.
Human cardiac troponin T: cloning and expression of new isoforms in the normal and failing heart.
TLDR
This work located the human cTnT gene by means of fluorescent in situ hybridization to 1q32 and, by sequencing thirteen cDNAs isolated from a human fetal heart cDNA library, identified three new isoforms resulting from specific combinations of three variable regions in human c TnT cDNA.
...
...