Structure, sequence and expression of the hepatitis delta (δ) viral genome

  title={Structure, sequence and expression of the hepatitis delta ($\delta$) viral genome},
  author={Kang Wang and Q-L. Choo and Amy J. Weiner and Jing-hsiung James Ou and R. C. Najarian and Richard M. Thayer and G. T. Mullenbach and Katherine J. Denniston and John L. Gerin and Michael Houghton},
Biochemical and electron microscopic data indicate that the human hepatitis δ viral agent contains a covalently closed circular and single-stranded RNA genome that has certain similarities with viroid-like agents from plants. The sequence of the viral genome (1,678 nucleotides) has been determined and an open reading frame within the complementary strand has been shown to encode an antigen that binds specifically to antisera from patients with chronic hepatitis δ viral infections. 

Organization and Expression of the Hepatitis Delta Virus Genome

Abstract The RNA genome of human hepatitis delta virus (HDV) is an unusual small circular single-stranded species that can fold on itself to form an unbranched rod-like structure. This RNA is

Cloning and sequencing of RNA of hepatitis delta virus isolated from human serum.

Comparison with the three complete published sequences shows that a region of the HDV genome, between positions 620 and 1350, which contains sequences involved in replication and possibly pathogenicity, is highly conserved.

Replication of hepatitis delta virus

Hepatitis delta virus is a unique viroid‐like human pathogen that is always associated with hepatitis B infection and the molecular basis for HDV pathogenicity remains uncertain.

A Cellular Homolog of Hepatitis Delta Antigen: Implications for Viral Replication and Evolution

Observations demonstrate that a host gene product can modulate HDV replication and suggest that HDV may have evolved from a primitive viroidlike RNA through capture of a cellular transcript.

Self-ligating RNA sequences on the antigenome of human hepatitis delta virus

A 179-base fragment of RNA from the 1,679-base antigenome of hepatitis delta virus can not only self-cleave but, when the ends of the resultant fragments are brought into apposition by base pairing

Editing on the genomic RNA of human hepatitis delta virus

A sensitive assay involving the polymerase chain reaction was developed to monitor changes on HDV RNA sequences as they occurred in transfected cells and found that the substrate for the sequence change was the viral genomic RNA rather than the antigenomic RNA.

The antigen of hepatitis delta virus: examination of in vitro RNA-binding specificity

Six different subregions of the HDV rodlike structure, three on the genomic RNA and three on its complement, the antigenomic RNA, were tested and found to be sufficient for antigen binding, but a similar structure present in the RNA of the potato spindle tuber viroid did not allow binding.

A specific base transition occurs on replicating hepatitis delta virus RNA

Three independent lines of evidence showed that when an infectious clone of hepatitis delta virus of known sequence was used to initiate genome replication, up to 41% of the genomes were specifically

Relating structure to function in the hepatitis delta virus antigen

Hepatitis delta virus expresses two forms of a single protein, the small (delta Ag-S) and large (delta Ag-L) antigens, which are identical except for an additional 19 residues present at the C



Cloned fragment of the hepatitis delta virus RNA genome: sequence and diagnostic application.

This cloned cDNA fragment has been used as a sensitive radioactive probe for the detection of HDV RNA in the serum of patients with either acute or chronic HDV infections.

Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli

The complete nucleotide sequence of hepatitis B virus genome (subtype ayw) cloned in Escherichia coli has been determined using the Maxam and Gilbert method and the dideoxynucleotide method. This

The hepatitis B virus

DNA recombinant technology has radically changed hepatitis B virus (HBV) virology. The genetic organization, transcription and replication of the virus are basically understood, structures of

Hop stunt viroid: molecular cloning and nucleotide sequence of the complete cDNA copy.

The complete cDNA of hop stunt viroid (HSV) has been cloned by the method of Okayama and Berg (1982) and the complete nucleotide sequence has been established.

delta Agent: association of delta antigen with hepatitis B surface antigen and RNA in serum of delta-infected chimpanzees.

A small RNA was temporally associated with delta antigen in the serum of infected chimpanzees and copurified with the delta-antigen-associated particles, smaller than the genomes of known RNA viruses but larger than the viroids of higher plants.

Comparative sequence and structure of viroid-like RNAs of two plant viruses.

A newly discovered group of spherical plant viruses contains a bipartite genome consisting of a single-strand linear RNA molecule, which possesses extensive intramolecular base pairing to give rod-like structures similar to those of viroids.

Delta hepatitis agent: structural and antigenic properties of the delta-associated particle

RNase susceptibility of the delta-associated RNA and release of delta Ag activity upon treatment of delta- associated particles with detergent revealed that this particle is organized into a virion-like form with the RNA and delta Ag as internal components within a coat of HBsAg.

The δ agent: HBsAg particles with δ antigen and RNA in the serum of an HBV carrier

It is demonstrated that delta antigen is associated with a unique subpopulation of hepatitis B surface antigen particles and small RNA in human serum.