Structure, expression and function of a schwannoma-derived growth factor

  title={Structure, expression and function of a schwannoma-derived growth factor},
  author={Hideo Kimura and Wolfgang H. Fischer and David R. Schubert},
DURING the development of the nervous system, cells require growth factors that regulate their division and survival. To identify new growth factors, serum-free growth-conditioned media from many clonal cell lines1 were screened for the presence of mitogens for central nervous system glial cells. A cell line secreting a potent glial mitogen was established from a tumour (or 'schwannoma') derived from the sheath of the sciatic nerve. The cells of the tumour, named JS1 cells, were adapted to… 
Schwannoma-derived growth factor promotes the neuronal differentiation and survival of PC12 cells
The present data show that SDGF causes the morphological and molecular differentiation of rat PC12 cells in a manner similar to, but distinguishable from nerve growth factor (NGF).
Axon-induced mitogenesis of human Schwann cells involves heregulin and p185erbB2.
Results imply that a HRG, or aHRG-like molecule, is a component of the axonal mitogen that induces proliferation through an interaction that involves p185erbB2 on Schwann cells.
Role for the stem cell factor/KIT complex in schwann cell neoplasia and mast cell proliferation associated with neurofibromatosis
The receptor for stem cell factor, KIT, was not expressed in normal Schwann cells but was expressed in the human Schwannoma line, suggesting that aberrant KIT expression may form an autocrine loop in certain Schwann cell neoplasias.
Conditioned media derived from glial cell lines promote survival and differentiation of dopaminergic neurons in vitro: Role of mesencephalic glia
All three media conditioned by several glial cell lines for neurotrophic effects on dopaminergic neurons in dissociated cell cultures were mitogenic for mesencephalic glia as demonstrated by glial fibrillary acidic protein (GFAP)‐immunocytochemistry in combination with [3H]thymidine‐autoradiography.
Expression of Neuregulins and their Putative Receptors, ErbB2 and ErbB3, Is Induced during Wallerian Degeneration
In contrast to the concept of NRGs as axon-associated mitogens, the findings suggest that NRGs produced by Schwann cells themselves may be partially responsible for Schwann cell proliferation during Wallerian degeneration, probably acting via autocrine or paracrine mechanisms.
Biological actions of the epidermal growth factors-like domain peptides of mouse schwannoma-derived growth factor and human amphiregulin.
It is found that SDGF(38-80) and AR(44-84) have weak mitogenic activity in NIH/3T3 cells and weak binding affinity to the EGF receptor on the surface of A431 cells compared with EGF, but results suggest that these two EGF-like peptides may be effective for neuronal differentiation rather than proliferation.
Glial growth factors I‐III are specific mitogens for glial cells
The characterization of the mitogenic and trophic activities for all three forms of GGF on rat Schwann cells and several other cell types are reported, consistent with the proposal that GGF‐I, G GF‐II, and GGF-III are a set of potent glial cell mitogens and putative ligands of members of the EGF receptor family, which may play important roles in the development, regeneration, and tumor biology of the peripheral nervous system.
Stem Cell Factor in Mast Cell and Schwann Cell Proliferation and Hyperplasia
This dissertation has cloned the gene encoding SCF from a eDNA library prepared from NIH 3T3 fibroblasts, and characterized the ability of recombinant SCF to induce the development of mast cell-committed progenitors (MCCP), found in the mesenteric lymph nodes of mice infected with Nippostrongylus brasiliensis {Nb-MLN).
Increased expression of Schwannoma‐derived growth factor (SDGF) mRNA in rat tumor cells: Involvement of SDGF in the growth promotion of rat gliomas
The results suggest that SDGF acts as an autocrine growth factor in the development and growth of rat tumors such as gliomas.


Release of autocrine growth factor by primary and immortalized Schwann cells.
It is found that repetitive passaging of Schwann cells with GGF and forskolin results in the loss of normal growth control; the cells are able to proliferate without added mitogens.
Identification and purification of glial growth factor
  • G. Lemke, J. Brockes
  • Biology
    The Journal of neuroscience : the official journal of the Society for Neuroscience
  • 1984
Purified human platelet-derived growth factor, a molecule with properties similar to those of GGF, is inactive on Schwann cells and therefore appears to be distinct.
Glial growth factor—like activity in Schwann cell tumors
It is concluded that acoustic neuromas contain a factor that is closely related to bovine pituitary GGF and that this factor may have a role in the abnormal proliferation of Schwann cells in these tumors.
Cloning and sequence analysis of a cDNA for rat transforming growth factor-α
The isolation of a complementary DNA clone encoding rat TGF-α, a 50-amino acid polypeptide that is present not only in retrovirus-transformed rat cells but also at lower levels in normal rat tissues, is reported.
Nucleotide sequence of epidermal growth factor cDNA predicts a 128,000-molecular weight protein precursor
The data indicate that the discrepancy between EGF levels in male and female mouse submaxillary glands (MSGs) is due to different EGF mRNA levels in these tissues, and suggest that precursor EGF processing may differ from that described previously for other polypeptide hormones.
The amphiregulin gene encodes a novel epidermal growth factor-related protein with tumor-inhibitory activity.
Human placenta and ovaries were found to express significant amounts of the 1.4-kilobase AR transcript, implicating AR in the regulation of normal cell growth, and the gene was localized to chromosomal region 4q13-4q21, a common breakpoint for acute lymphoblastic leukemia.
Receptor- and heparin-binding domains of basic fibroblast growth factor.
The results suggest the possibility of designing specific analogs of FGF that are capable of inhibiting the biological effects of FGRF, and specifically those that possess partial agonist activity and stimulate DNA synthesis when tested in the absence of exogenous FGF.
Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue
These preparations should significantly aid in efforts to examine the biochemistry, physiology, and pharmacology of these two major classes of central nervous system cells.
Amphiregulin: a bifunctional growth-modulating glycoprotein produced by the phorbol 12-myristate 13-acetate-treated human breast adenocarcinoma cell line MCF-7.
The amino-terminal amino acid sequence of AR has been determined, and no significant sequence homology between AR and other proteins was found, and the molecule thus appears to be a distinct growth regulatory protein.