Structure, composition, and maturation of newly deposited calcium-phosphate crystals in chicken osteoblast cell cultures.

@article{Kuhn2000StructureCA,
  title={Structure, composition, and maturation of newly deposited calcium-phosphate crystals in chicken osteoblast cell cultures.},
  author={Liisa T. Kuhn and Yaotang Wu and Christian C. Rey and Louis C. Gerstenfeld and Marc D. Grynpas and Jerome L. Ackerman and H. M. Kim and Melvin J. Glimcher},
  journal={Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research},
  year={2000},
  volume={15 7},
  pages={
          1301-9
        }
}
Characterization of the very early calcium phosphate (CaP) crystals deposited in bone or in osteoblast cell cultures has been hampered by the overwhelming presence of organic matrix components and cells that obscure spectral analyses. We have overcome this problem using isolated protein-free crystals and have obtained new data including 31P nuclear magnetic resonance (NMR) spectra for the first time from mineral crystals deposited during osteoblast calcification in culture. Crystals were… CONTINUE READING

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Ultrastructural and Mineral Phase Characterization of the Bone-Like Matrix Assembled in F-OST Osteoblast Cultures

  • Calcified Tissue International
  • 2011
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