Structural transformation of lignan compounds in rat gastrointestinal tract.

  title={Structural transformation of lignan compounds in rat gastrointestinal tract.},
  author={Mitsuhiko Nose and Toru Fujimoto and Tadahiro Takeda and Sansei Nishibe and Yukio Ogihara},
  journal={Planta medica},
  volume={58 6},
Structural transformation of arctiin and tracheloside, major components of seeds of Arctium lappa and Carthamus tinctorius, were investigated using rat gastric juice (pH 1.2-1.5) and rat large intestinal flora in vitro. Quantitative analysis of lignans and their metabolites was carried out by high performance liquid chromatography. Both lignans were stable in rat gastric juice and arctiin was rapidly transformed to arctigenin in rat large intestinal flora, followed by conversion to the major… 

Simultaneous determination of arctiin and its metabolites in rat urine and feces by HPLC.

Study on human intestinal bacterium Blautia sp. AUH-JLD56 for the conversion of arctigenin to (-)-3'-desmethylarctigenin.

The biotransforming kinetics and maximum biOTransforming capacity of strain AUH-JLD56 was investigated and the metabolite 3'-DMAG showed significantly higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity than that of the substrate arctigenin at the concentrations tested.

Transformation of arctiin to estrogenic and antiestrogenic substances by human intestinal bacteria.

The results indicate that the transformation of 1 by intestinal flora might be essential for the manifestation of the estrogenic and antiestrogenic activity of 1.

Anti-austeric Activity of Phenolic Constituents of Seeds of Arctium lappa

The in vitro preferential cytotoxic activity of these pure compounds and 1:1 mixtures, together with enterodiol and enterolactone, against human pancreatic cancer PANC-1 cells in nutrient-deprived medium (NDM) was evaluated.

Ligand Binding Affinities of Arctigenin and Its Demethylated Metabolites to Estrogen Receptor Alpha

Ligand binding affinities of (−)-arctigenin and its seven metabolites, including DHENL, were investigated for an estrogen receptor alpha, and it was found that demethylated metabolites had stronger bindingAffinities than (−)-Arctigen in using a ligand binding screen assay method.

Mammalian phytoestrogens: enterodiol and enterolactone.

  • Li-Quan Wang
  • Biology, Medicine
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
  • 2002

Isolation and Characterization of a Human Intestinal Bacterium Eggerthella sp. AUH-JLD49s for the Conversion of (-)-3'-Desmethylarctigenin.

The metabolite of 3'-DMAG by strain AUH-JLD49s was identified as 3'-desmethyl-4'-dehydroxyarctigenin (DMDH-AG) based on electrospray ionization mass spectrometry and 1H and 13C nuclear magnetic resonance spectroscopy and showed an inhibitory effect on cell growth of human colon cancer cell lines HCT116 and human breast cancer cell line MDA-MB-231.