Structural studies on rabbit skeletal muscle actin. Ordering of the peptides produced by cleavage with cyanogen bromide.

@article{Kuehl1975StructuralSO,
  title={Structural studies on rabbit skeletal muscle actin. Ordering of the peptides produced by cleavage with cyanogen bromide.},
  author={Walter Michael Kuehl and Mary Anne Conti and Robert S Adelstein},
  journal={The Journal of biological chemistry},
  year={1975},
  volume={250 15},
  pages={5890-6}
}
The 17 peptides produced by cleavage of actin with cyanogen bromide have been ordered with regard to their sequence in the actin molecule. Tryptic digestion of actin followed by isolation of the methionine-containing "overlap" peptides permitted the unique alignment of most, but not all of the cyanogen bromide peptides. However, maleylation of the actin molecule followed by tryptic digestion and isolation of methionine-containing peptides from maleylated actin permitted the proper placement of… CONTINUE READING