Structural studies on cutinase, a glycoprotein containing novel amino acids and glucuronic acid amide at the N terminus.

@article{Lin1980StructuralSO,
  title={Structural studies on cutinase, a glycoprotein containing novel amino acids and glucuronic acid amide at the N terminus.},
  author={Thomas H S Lin and Pappachan E. Kolattukudy},
  journal={European journal of biochemistry},
  year={1980},
  volume={106 2},
  pages={341-51}
}
Cutinase I and cutinase II, two extracellular enzymes produced by Fusarium solani pisi, were shown to be glycoproteins containing 4.3% and 5.1% carbohydrates, respectively. Upon treatment with alkali both enzymes generated chromophores which absorbed at 241 nm. Treatment of both proteins with alkaline NaB3H4 gave labeled protein and labeled monosaccharides. Hydrolysis of the labeled protein followed by chromatographic and enzymatic analyses of the products showed that alanine, 2-aminobutyrate… CONTINUE READING