Structural specificity in a FGF7-affinity purified heparin octasaccharide required for formation of a complex with FGF7 and FGFR2IIIb.

@article{Luo2006StructuralSI,
  title={Structural specificity in a FGF7-affinity purified heparin octasaccharide required for formation of a complex with FGF7 and FGFR2IIIb.},
  author={Yongde Luo and Sheng Ye and Mikio Kan and Wallace L. Mckeehan},
  journal={Journal of cellular biochemistry},
  year={2006},
  volume={97 6},
  pages={
          1241-58
        }
}
  • Yongde Luo, Sheng Ye, +1 author Wallace L. Mckeehan
  • Published in
    Journal of cellular…
    2006
  • Biology, Medicine
  • Variations in sulfation of heparan sulfate (HS) affect interaction with FGF, FGFR, and FGF-HS-FGFR signaling complexes. Whether structurally distinct HS motifs are at play is unclear. Here we used stabilized recombinant FGF7 as a bioaffinity matrix to purify size-defined heparin oligosaccharides. We show that only 0.2%-4% of 6 to 14 unit oligosaccharides, respectively, have high affinity for FGF7 based on resistance to salt above 0.6M NaCl. The high affinity fractions exhibit highest specific… CONTINUE READING

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