Structural specificities of five commonly used DNA nucleases.

  title={Structural specificities of five commonly used DNA nucleases.},
  author={Horace R. Drew},
  journal={Journal of molecular biology},
  volume={176 4},
Five commonly used nucleases were surveyed for their ability to distinguish among several different DNA backbone configurations. The digestion data suggest that: (1) DNAase I binds across the minor groove; whereas (2) nuclease S1 and (3) micrococcal nuclease bind to an exposed single strand; (4) copper/phenanthroline seeks a base-pair step; and (5) DNAase II requires just a stacked single strand of limited exposure. Only micrococcal nuclease is demonstrably base-specific, with a strong… CONTINUE READING

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