Structural snapshots of the reaction coordinate for O-GlcNAc transferase

Abstract

Visualization of the reaction coordinate undertaken by glycosyltransferases has remained elusive but is critical for understanding this important class of enzyme. Using substrates and substrate mimics, we describe structural snapshots of all species along the kinetic pathway for human O-linked β-N-acetylglucosamine transferase (O-GlcNAc transferase), an intracellular enzyme that catalyzes installation of a dynamic post-translational modification. The structures reveal key features of the mechanism and show that substrate participation is important during catalysis.

DOI: 10.1038/nchembio.1109

Extracted Key Phrases

01020201520162017
Citations per Year

Citation Velocity: 6

Averaging 6 citations per year over the last 3 years.

Learn more about how we calculate this metric in our FAQ.

Cite this paper

@inproceedings{Lazarus2012StructuralSO, title={Structural snapshots of the reaction coordinate for O-GlcNAc transferase}, author={Michael B. Lazarus and Jiaoyang Jiang and Tracey M. Gloster and Wesley F. Zandberg and Garrett E. Whitworth and David J Vocadlo and Suzanne Walker}, booktitle={Nature chemical biology}, year={2012} }