Structural snapshots of the reaction coordinate for O-GlcNAc transferase


Visualization of the reaction coordinate undertaken by glycosyltransferases has remained elusive but is critical for understanding this important class of enzyme. Using substrates and substrate mimics, we describe structural snapshots of all species along the kinetic pathway for human O-linked β-N-acetylglucosamine transferase (O-GlcNAc transferase), an intracellular enzyme that catalyzes installation of a dynamic post-translational modification. The structures reveal key features of the mechanism and show that substrate participation is important during catalysis.

DOI: 10.1038/nchembio.1109

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@inproceedings{Lazarus2012StructuralSO, title={Structural snapshots of the reaction coordinate for O-GlcNAc transferase}, author={Michael B. Lazarus and Jiaoyang Jiang and Tracey M. Gloster and Wesley F. Zandberg and Garrett E. Whitworth and David J Vocadlo and Suzanne Walker}, booktitle={Nature chemical biology}, year={2012} }