Structural similarities between M. luteus and E. coli DNA topoisomerase I.

Abstract

Type I DNA topoisomerase has been isolated from Micrococcus luteus following a procedure that takes advantage of the binding of the enzyme to heparin and single stranded DNA. Almost pure enzyme was obtained using two successive affinity chromatography steps: on heparin Sepharose and ssDNA cellulose. The method was rapid and allowed the isolation of DNA… (More)

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@article{Anderluzzi1993StructuralSB, title={Structural similarities between M. luteus and E. coli DNA topoisomerase I.}, author={Daniela Anderluzzi and Antonia Maria Pedrini}, journal={Biochemical and biophysical research communications}, year={1993}, volume={192 2}, pages={657-64} }