Structural requirements of Escherichia coli formylmethionyl transfer ribonucleic acid for ribosome binding and initiation of protein synthesis.

Abstract

Escherichia coli formylmethionine tRNA can be isolated as two isomers which differ by a single nucleotide in the variable loop (m7G uersus adenosine). These two initiator tRNAs have been found to bind to ribosomes in an initiation factor and AUG-dependent reaction with identical kinetics. In addition, the bound tRNAs react with puromycin and initiate… (More)

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