Structural requirements of Escherichia coli formylmethionyl transfer ribonucleic acid for ribosome binding and initiation of protein synthesis.


Escherichia coli formylmethionine tRNA can be isolated as two isomers which differ by a single nucleotide in the variable loop (m7G uersus adenosine). These two initiator tRNAs have been found to bind to ribosomes in an initiation factor and AUG-dependent reaction with identical kinetics. In addition, the bound tRNAs react with puromycin and initiate… (More)


5 Figures and Tables