Structural requirements for in vivo detection of cell death with 99mTc-annexin V.

@article{Tait2005StructuralRF,
  title={Structural requirements for in vivo detection of cell death with 99mTc-annexin V.},
  author={Jonathan F. Tait and Christina B Smith and Francis Blankenberg},
  journal={Journal of nuclear medicine : official publication, Society of Nuclear Medicine},
  year={2005},
  volume={46 5},
  pages={807-15}
}
UNLABELLED (99m)Tc-Annexin V is used to image cell death in vivo via high-affinity binding to exposed phosphatidylserine. We investigated how changes in membrane-binding affinity, molecular charge, and method of labeling affected its biodistribution in normal mice and its uptake in apoptotic tissues. METHODS An endogenous Tc chelation site (Ala-Gly-Gly-Cys-Gly-His) was added to the N-terminus of annexin V to create annexin V-128. The membrane-binding affinity of annexin V-128 was then… CONTINUE READING

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