Structural rearrangements of the 10-23 DNAzyme to beta 3 integrin subunit mRNA induced by cations and their relations to the catalytic activity.

@article{Cielak2003StructuralRO,
  title={Structural rearrangements of the 10-23 DNAzyme to beta 3 integrin subunit mRNA induced by cations and their relations to the catalytic activity.},
  author={Marcin J Cieślak and Jacek Szymanski and Ryszard W. Adamiak and Czeslaw S. Cierniewski},
  journal={The Journal of biological chemistry},
  year={2003},
  volume={278 48},
  pages={47987-96}
}
The intracellular ability of the "10-23" DNAzyme to efficiently inhibit expression of targeted proteins has been evidenced by in vitro and in vivo studies. However, standard conditions for kinetic measurements of the DNAzyme catalytic activity in vitro include 25 mM Mg2+, a concentration that is very unlikely to be achieved intracellularly. To study this discrepancy, we analyzed the folding transitions of the 10-23 DNAzyme induced by Mg2+. For this purpose, spectroscopic analyzes such as… CONTINUE READING

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