Structural plasticity of hippocampal mossy fiber synapses as revealed by high-pressure freezing.

@article{Zhao2012StructuralPO,
  title={Structural plasticity of hippocampal mossy fiber synapses as revealed by high-pressure freezing.},
  author={Shanting Zhao and Daniel Studer and Xuejun Chai and Werner Graber and Nils Brose and Sigrun Nestel and Christina C Young and E. Patricia Rodriguez and Kurt S{\"a}tzler and Michael Frotscher},
  journal={The Journal of comparative neurology},
  year={2012},
  volume={520 11},
  pages={2340-51}
}
Despite recent progress in fluorescence microscopy techniques, electron microscopy (EM) is still superior in the simultaneous analysis of all tissue components at high resolution. However, it is unclear to what extent conventional fixation for EM using aldehydes results in tissue alteration. Here we made an attempt to minimize tissue alteration by using rapid high-pressure freezing (HPF) of hippocampal slice cultures. We used this approach to monitor fine-structural changes at hippocampal mossy… CONTINUE READING

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