Structural origins of substrate discrimination in trypsin and chymotrypsin.

  title={Structural origins of substrate discrimination in trypsin and chymotrypsin.},
  author={John J Perona and Lizbeth Hedstrom and William J. Rutter and Robert Fletterick},
  volume={34 5},
Converting the specificity of trypsin to that of chymotrypsin has been shown to require the exchange of amino acids in multiple portions of the protein, including two surface loops which do not directly contact the substrate. Crystallographic analysis of two mutant trypsins possessing chymotrypsin-like specificity now reveals that these distal surface loops alter function by directly determining the structure of the primary binding site. Efficient acylation of cognate substrates correlates with… CONTINUE READING

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