Structural origins of pH-dependent chemical shifts in the B1 domain of protein G.

@article{Tomlinson2010StructuralOO,
  title={Structural origins of pH-dependent chemical shifts in the B1 domain of protein G.},
  author={Jennifer H Tomlinson and Victoria L Green and Patrick J Baker and Michael P Williamson},
  journal={Proteins},
  year={2010},
  volume={78 14},
  pages={
          3000-16
        }
}
We report chemical shifts for H(N), N, and C' nuclei in the His-tagged B1 domain of protein G (GB1) over a range of pH values from pH 2.0 to 9.0, which fit well to standard pH-dependent equations. We also report a 1.2 Å resolution crystal structure of GB1 at pH 3.0. Comparison of this crystal structure with published crystal structures at higher pHs provides details of the structural changes in GB1 associated with protonation of the carboxylate groups, in particular a conformational change in… CONTINUE READING

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