Structural insights into biosynthesis of resorcinolic lipids by a type III polyketide synthase in Neurospora crassa.

@article{Goyal2008StructuralII,
  title={Structural insights into biosynthesis of resorcinolic lipids by a type III polyketide synthase in Neurospora crassa.},
  author={Aneesh Goyal and Priti Saxena and Ataur Rahman and Parmit Kumar Singh and Durgadas P. Kasbekar and Rajesh S. Gokhale and Rajan Sankaranarayanan},
  journal={Journal of structural biology},
  year={2008},
  volume={162 3},
  pages={
          411-21
        }
}
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References

SHOWING 1-10 OF 65 REFERENCES
A novel tunnel in mycobacterial type III polyketide synthase reveals the structural basis for generating diverse metabolites
TLDR
The crystal structure of PKS18 reveals a 20 Å substrate binding tunnel, hitherto unidentified in this superfamily of enzymes, which underlines a fascinating example of the way in which subtle changes in protein architecture can generate metabolite diversity in nature.
A New Family of Type III Polyketide Synthases in Mycobacterium tuberculosis*
TLDR
It is proposed that these proteins might have evolved by fusing the catalytic machinery of CHS and β-ketoacyl synthases, the two evolutionarily related members with conserved thiolase fold, to provide a fascinating example of metabolite divergence in CHS-like proteins.
Crystal Structure of a Bacterial Type III Polyketide Synthase and Enzymatic Control of Reactive Polyketide Intermediates*
TLDR
The crystal structure of Streptomyces coelicolor THNS reveals an unanticipated novel cavity extending into the “floor” of the traditional active site cavity, providing the first plausible structural and mechanistic explanation for yet another unusual THNS catalytic activity: its previously inexplicable extra polyketide extension step when primed with a long acyl starter.
The chalcone synthase superfamily of type III polyketide synthases.
TLDR
A major focus of this review is the integration of information from genetic and biochemical studies with the unique insights gained from protein X-ray crystallography and homology modeling, which generated a number of new predictions regarding both the importance and mechanistic role of various amino acid substitutions observed among functionally diverse type III PKS enzymes.
Macrotetrolide biosynthesis: a novel type II polyketide synthase.
TLDR
Genetic analysis of the macrotetrolide biosynthesis unveiled an unprecedented organization for a polyketide gene cluster that features five genes encoding discrete ketoacyl synthase (KS) and four genes encode discrete ketoreductase (KR) but lacking an acyl carrier protein (ACP).
Pentaketide Resorcylic Acid Synthesis by Type III Polyketide Synthase from Neurospora crassa*
TLDR
ORAS is the first type III PKS that synthesizes pentaketide resorcylic acids, and aldol condensation and aromatization of the intermediate, which is still attached to the enzyme, are presumably followed by hydrolysis for release of the product as a resorCylic acid.
An aldol switch discovered in stilbene synthases mediates cyclization specificity of type III polyketide synthases.
Versatility of polyketide synthases in generating metabolic diversity.
Versatile polyketide enzymatic machinery for the biosynthesis of complex mycobacterial lipids.
TLDR
The focus of the Highlight is to emphasize the functional role of polyketide synthase (PKS) proteins in the biosynthesis of complex mycobacterial lipids and the catalytic as well as mechanistic versatility of PKS.
Structure of chalcone synthase and the molecular basis of plant polyketide biosynthesis
TLDR
The three-dimensional structure of CHS elucidates the chemical basis of plant polyketide biosynthesis and provides a framework for engineering CHS-like enzymes to produce new products.
...
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