Structural features of the binding site of cholera toxin inferred from fluorescence measurements.

@article{Wolf1985StructuralFO,
  title={Structural features of the binding site of cholera toxin inferred from fluorescence measurements.},
  author={M. D. De Wolf and G. V. Van Dessel and A. Lagrou and H. Hilderson and W. Dierick},
  journal={Biochimica et biophysica acta},
  year={1985},
  volume={832 2},
  pages={
          165-74
        }
}
The dependence on pH of the fluorescence of cholera toxin and its A and B subunits has been studied at 25 degrees C. The fluorescence intensity of cholera toxin is highly pH-dependent. In the pH range 7-9.5 it reaches a maximum corresponding to a quantum yield of 0.076. In the pH range 4-7 a strong increase in fluorescence intensity is observed (delta Q/Qmax = 0.64). Evaluation of the pH sensitivity of the fluorescence intensity of the A and B subunits reveals that the B subunit is mainly… Expand
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