Structural features of cultured epithelial cells from the adult rat epididymis.

  title={Structural features of cultured epithelial cells from the adult rat epididymis.},
  author={Gary E Olson and J. Jonas-Davies and Loren H. Hoffman and Marie-Claire Orgebin-Crist},
  journal={Journal of andrology},
  volume={4 6},
Epithelial cells isolated from the caput epididymidis of adult rats were placed in primary culture and examined daily for ten days for changes in external anatomy, reorganization of cytoskeletal components, maintenance of characteristic cytoplasmic features, and response to media formulated to minimize nonepithelial cell proliferation. Significant cell attachment to the substrate began after the first 24 hours of culture. After attachment, the cells underwent a progressive flattening and became… CONTINUE READING

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