Structural features of cephalosporin acylase reveal the basis of autocatalytic activation.

@article{Cho2009StructuralFO,
  title={Structural features of cephalosporin acylase reveal the basis of autocatalytic activation.},
  author={Ki Joon Cho and Jin Kwang Kim and Ji-Hye Lee and Hye Jeong Shin and Sung Soo Park and Kyung Hyun Kim},
  journal={Biochemical and biophysical research communications},
  year={2009},
  volume={390 2},
  pages={342-8}
}
Cephalosporin acylase (CA), a member of the N-terminal nucleophile hydrolase family, is activated through two steps of intramolecular autoproteolysis, the first mediated by a serine residue, and the second by a glutamate, which releases the pro-segment and produces an active enzyme. In this study, we have determined the crystal structures of mutants which… CONTINUE READING