Structural elucidation of the disulfated oligosaccharide from bovine lutropin.

  title={Structural elucidation of the disulfated oligosaccharide from bovine lutropin.},
  author={Eric D. Green and Herman van Halbeek and Irving Boime and Jacques U. Baenziger},
  journal={The Journal of biological chemistry},
  volume={260 29},
The Asn-linked oligosaccharides of the pituitary hormone lutropin (LH) contain both sulfate and GalNAc. Bovine pituitary explants incorporate [3H]glucosamine, [3H]mannose, [3H]fucose, and [35S]sulfate into the Asn-linked oligosaccharides of LH. Endoglycosidase F or N-glycanase releases the [3H]glucosamine- and [3H]mannose-labeled oligosaccharides from the protein, which resolve on anion-exchange high pressure liquid chromatography as neutral (S-0), mono- (S-1), and disulfated (S-2) species… CONTINUE READING
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