Structural elucidation of SrtA enzyme in Enterococcus faecalis: an emphasis on screening of potential inhibitors against the biofilm formation.

@article{Selvaraj2014StructuralEO,
  title={Structural elucidation of SrtA enzyme in Enterococcus faecalis: an emphasis on screening of potential inhibitors against the biofilm formation.},
  author={Chandrabose Selvaraj and Jeyachandran Sivakamavalli and Baskaralingam Vaseeharan and Poonam Singh and Sanjeev Kumar Singh},
  journal={Molecular bioSystems},
  year={2014},
  volume={10 7},
  pages={
          1775-89
        }
}
Enterococcus faecalis is a pathogenic Gram-positive bacterium, which mainly infects humans through urinary tract infections. SrtA is an essential enzyme for survival of E. faecalis, and inhibition of this particular enzyme will reduce the virulence of biofilm formation. It is proved to be associated with the microbial surface protein embedded signal transduction mechanism and promising as a suitable anti-microbial drug target for E. faecalis. The present work gives an inclusive description of… 

Tables from this paper

Screening of curcumin analogues targeting Sortase A enzyme of Enterococcus faecalis: a molecular dynamics approach

TLDR
From the molecular dynamics results, it is proposed that the analogue CA51 is a potent inhibitor of E. faecalis SrtA and can be used as novel antimicrobial compound against E. Faecalis infections.

Dissecting the structure–function relationship in lysozyme domain of mycobacteriophage D29‐encoded peptidoglycan hydrolase

TLDR
LD is presented as a potent candidate for developing phage‐based broad‐spectrum therapeutics for rapidly emerging drug‐resistant bacteria such as Mycobacterium tuberculosis by characterizing an exhaustive LD protein variant library and identifying critical residues important for LD activity and stability.

Antibacterial of Dibenzo-p-Dioxi-2,8-Dicarboxylic Acid Against Pathogenic Oral Bacteria E. faecalis ATCC 29212 Peptide Pheromones: Quorum Sensing of in vitro and in silico Study

TLDR
It is proposed that compound 1 has a good activity to inhibit E. faecalis through its peptide pheromones in the QS system.

Molecular Insights on exquisitely Selective SrtA inhibitors towards active site loop forming open/close lid conformations in SrtA from Bacillus anthracis

TLDR
The present study clearly explains the dependency of inhibitory activities in SrtA inhibitors is closely related to protein conformational changes of SrtB from Bacillus anthracis B and this work may lead to a better understanding of the mechanism of action and aid to design a novel and more potent SRTA inhibitors.

Effectiveness of Bioactive Compound as Antibacterial and Anti-Quorum Sensing Agent from Myrmecodia pendans: An In Silico Study

TLDR
This study aims to investigate and predict the action mode of antibacterial compounds with specific proteins by following the molecular docking approach and finds that compound 2 can be a lead compound for an antibacterial agent in each pathway.

High-Throughput Screening and Quantum Mechanics for Identifying Potent Inhibitors Against Mac1 Domain of SARS-CoV-2 Nsp3

TLDR
Overall binding phenomenon shows both substrate and lead molecules are well-adopt to bind with similar binding mode inside the closed form of Mac1, which makes it an ideal drug target and inhibitors developed against them can possess a broad antiviral activity against CoV.

Structure-Based Virtual Screening and Biological Evaluation of a Calpain Inhibitor for Prevention of Selenite-Induced Cataractogenesis in an in Vitro System

TLDR
A potent lead compound is demonstrated, HTS08688, that exhibits putative anticataractogenic activity by virtue of its potential to inhibit calpain.

References

SHOWING 1-10 OF 62 REFERENCES

Identification of surface proteins in Enterococcus faecalis V583

TLDR
The present proteome analysis gives an experimental impression of the protein landscape on the cell surface of the pathogenic bacterium E. faecalis, and several proteins involved in cell wall synthesis, pheromone-regulated processes, and transport of solutes are identified.

Contribution of Autolysin and Sortase A during Enterococcus faecalis DNA-Dependent Biofilm Development

TLDR
A role for Atn is demonstrated in the pathway leading to DNA release during biofilm development in E. faecalis and it is shown to be particularly important during the accumulative phase for maturation and architectural stability of biofilms.

Enterococcus faecalis Prophage Dynamics and Contributions to Pathogenic Traits

TLDR
Prophages encoding platelet-binding-like proteins were found to be involved in adhesion to human platelets, considered as a first step towards the development of infective endocarditis, and provide an explanation for the correlation between antibiotic usage and E. faecalis success as a nosocomial pathogen.

Validation of potential inhibitors for SrtA against Bacillus anthracis by combined approach of ligand-based and molecular dynamics simulation

TLDR
The dynamic state of interaction and binding energy assess that new compounds are more active inside the binding pocket and these compounds on experimental validations will survive as better inhibitors for targeting the cell adhesion mechanism of Ba-SrtA.

Exploration of fluoroquinolone resistance in Streptococcus pyogenes: comparative structure analysis of wild‐type and mutant DNA gyrase

TLDR
The present study unravels the fact that mutation at the drug binding site is the major cause for different level of resistance by the S. pyogenes when exposed against the varying concentrations of the fluoroquinolones.

Efflux Pump Inhibitor Potentiates Antimicrobial Photodynamic Inactivation of Enterococcus faecalis Biofilm

TLDR
These experiments demonstrated the advantage of a cationic phenothiazinium photosensitizer combined with an EPI to inactivate biofilm bacteria and disrupt biofilm structure.

Biofilm formation and esp gene carriage in enterococci

TLDR
The acquisition of vancomycin resistance may result in a lower ability to form biofilms, but a larger study using clinical isolates is needed to test this hypothesis.

Development of a Method for Markerless Genetic Exchange in Enterococcus faecalis and Its Use in Construction of a srtA Mutant

TLDR
The development of a counterselectable markerless genetic exchange system based on the upp-encoded uracil phosphoribosyl transferase of E. faecalis is reported, which will facilitate detailed genetic analysis of these important pathogens.

Relevance and application of sortase and sortase-dependent proteins in lactic acid bacteria

TLDR
This review examines the collection of studies which evaluate sortase and SDPs in select species of dairy-associated and health promoting LAB and sheds light on their important roles in mediating nutrient acquisition through proteinase P as well as positive probiotic attributes.

Antimicrobial actions of benzimidazoles against oral streptococci.

TLDR
Benzimidazoles appear to be useful inhibitors of oral bacteria in acid environments such as progressing caries lesions.
...