Structural dynamics and topology of phospholamban in oriented lipid bilayers using multidimensional solid-state NMR.

@article{Traaseth2006StructuralDA,
  title={Structural dynamics and topology of phospholamban in oriented lipid bilayers using multidimensional solid-state NMR.},
  author={Nathaniel J Traaseth and Jarrod J Buffy and Jamillah Zamoon and Gianluigi Veglia},
  journal={Biochemistry},
  year={2006},
  volume={45 46},
  pages={13827-34}
}
Phospholamban (PLN), a single-pass membrane protein, regulates heart muscle contraction and relaxation by reversible inhibition of the sarco(endo)plasmic reticulum Ca-ATPase (SERCA). Studies in detergent micelles and oriented lipid bilayers have shown that in its monomeric form PLN adopts a dynamic L shape (bent or T state) that is in conformational equilibrium with a more dynamic R state. In this paper, we use solid-state NMR on both uniformly and selectively labeled PLN to refine our initial… CONTINUE READING