Structural dynamics and functional domains of the fur protein.

  title={Structural dynamics and functional domains of the fur protein.},
  author={Mark Coy and John B. Neilands},
  volume={30 33},
Proteolytic enzymes were used to detect metal-induced conformational changes in the ferric uptake regulation (Fur) protein of Escherichia coli K12. Metal binding results in enhanced cleavage of the N-terminal region of Fur by trypsin and chymotrypsin. Activation of both trypsinolysis sensitivity and DNA binding have similar metal ion specificity and concentration dependencies, suggesting that the conformational change detected is required for operator DNA binding. Isolation and characterization… CONTINUE READING

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