Structural determination of sphingomyelin by tandem mass spectrometry with electrospray ionization.

@article{Hsu2000StructuralDO,
  title={Structural determination of sphingomyelin by tandem mass spectrometry with electrospray ionization.},
  author={Fong-Fu Hsu and John Turk},
  journal={Journal of the American Society for Mass Spectrometry},
  year={2000},
  volume={11 5},
  pages={437-49}
}
Alkaline metal adduct ions of sphingomyelin were formed by electrospray ionization in positive ion mode. Under low energy collisionally activated dissociation (CAD), the product ion spectra yield abundant fragment ions representative of both long chain base and fatty acid which permit unequivocal determination of the structure. Tandem spectra obtained by constant neutral loss scanning permit identification of sphingomyelin class and specific long chain base subclass in the mixture. The… CONTINUE READING