Structural determinants of glutathione transferases with azathioprine activity identified by DNA shuffling of alpha class members.

@article{Kurtovic2008StructuralDO,
  title={Structural determinants of glutathione transferases with azathioprine activity identified by DNA shuffling of alpha class members.},
  author={Sanela Kurtovic and Olof Mod{\'e}n and Abeer Shokeer and Bengt Mannervik},
  journal={Journal of molecular biology},
  year={2008},
  volume={375 5},
  pages={1365-79}
}
A library of alpha class glutathione transferases (GSTs), composed of chimeric enzymes derived from human (A1-1, A2-2 and A3-3), bovine (A1-1) and rat (A2-2 and A3-3) cDNA sequences was constructed by the method of DNA shuffling. The GST variants were screened in bacterial lysates for activity with the immunosuppressive agent azathioprine, a prodrug that is transformed into its active form, 6-mercaptopurine, by reaction with the tripeptide glutathione catalyzed by GSTs. Important structural… CONTINUE READING

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