Structural determinants of cytochrome P450 2B1 specificity: evidence for five substrate recognition sites.

@article{He1994StructuralDO,
  title={Structural determinants of cytochrome P450 2B1 specificity: evidence for five substrate recognition sites.},
  author={You Ai He and Zuming Luo and P A Klekotka and Vicki L. Burnett and James R. Halpert},
  journal={Biochemistry},
  year={1994},
  volume={33 14},
  pages={4419-24}
}
Twelve site-directed mutants of rat cytochrome P450 2B1 distributed over seven positions and four putative substrate recognition sites (SRS) were constructed and expressed in COS cells. Function was examined using androstenedione and testosterone as substrates. Substitutions at positions 303, 360, and 473 did not markedly affect the regio- or stereoselectivity of androgen metabolism, whereas mutants in positions 206 (SRS-2), 302 (SRS-4), and 363 and 367 (SRS-5) exhibited markedly different… CONTINUE READING