Structural determinants of conformationally selective, prion-binding aptamers.

  title={Structural determinants of conformationally selective, prion-binding aptamers.},
  author={Natalie M Sayer and Matthew Cubin and Alexandre G O Rhie and Marc David Bullock and Abdessamad Tahiri-Alaoui and William James},
  journal={The Journal of biological chemistry},
  volume={279 13},
We have recently described the isolation of 2'-fluoropyrimidine-substituted RNA aptamers that bind selectively to disease-associated beta-sheet-rich forms of the prion protein, PrP, from a number of mammalian species. These aptamers inhibit the accumulation of protease-resistant forms of PrP in a prion-seeded, in vitro conversion assay. Here we identify the minimal portions of two of these aptamers that retain binding specificity. We determine their secondary structures by a combination of… CONTINUE READING