Structural determinants of Ras-Raf interaction analyzed in live cells.

  title={Structural determinants of Ras-Raf interaction analyzed in live cells.},
  author={Tzvetanka Bondeva and Andr{\'a}s Balla and P{\'e}ter V{\'a}rnai and Tamas Balla},
  journal={Molecular biology of the cell},
  volume={13 7},
The minimum structure of the Raf-1 serine/threonine kinase that recognizes active Ras was used to create a green fluorescent fusion protein (GFP) for monitoring Ras activation in live cells. In spite of its ability to bind activated Ras in vitro, the Ras binding domain (RBD) of Raf-1 (Raf-1[51-131]GFP) failed to detect Ras in Ras-transformed NIH 3T3 fibroblasts and required the addition of the cysteine-rich domain (CRD) (Raf-1[51-220]GFP) to show clear localization to plasma membrane ruffles… CONTINUE READING


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