Structural comparison of recombinant human macrophage colony stimulating factor beta and a partially reduced derivative using hydrogen deuterium exchange and electrospray ionization mass spectrometry.

@article{Zhang2001StructuralCO,
  title={Structural comparison of recombinant human macrophage colony stimulating factor beta and a partially reduced derivative using hydrogen deuterium exchange and electrospray ionization mass spectrometry.},
  author={Y Heidi Zhang and Xuguang Yan and Claudia S. Maier and Michael I Schimerlik and Max L Deinzer},
  journal={Protein science : a publication of the Protein Society},
  year={2001},
  volume={10 11},
  pages={2336-45}
}
Hydrogen deuterium exchange, monitored by electrospray ionization mass spectrometry, has been employed to characterize structural features of a derivative of recombinant human macrophage colony stimulating factor beta (rhm-CSFbeta) in which two of the nine disulfide bridges (Cys157/Cys159-Cys'157/Cys'159) were selectively reduced and alkylated. Removal of… CONTINUE READING