Structural comparison of purified dynein proteins with in situ dynein arms.

@article{Goodenough1984StructuralCO,
  title={Structural comparison of purified dynein proteins with in situ dynein arms.},
  author={Ursula W. Goodenough and John E. Heuser},
  journal={Journal of molecular biology},
  year={1984},
  volume={180 4},
  pages={1083-118}
}
Using the quick-freeze deep-etch technique, we describe the structure of outerarm dynein proteins from Chlamydomonas and Tetrahymena after adsorption to a mica surface, after high-salt dissociation, and after glutaraldehyde fixation, and compare these images to the configuration of outer arms bound to microtubules. After adsorption to mica, the extracted dyneins from both organisms look like three-headed "bouquets", as reported for Tetrahymena by Johnson & Wall (1983b). High magnification… CONTINUE READING