Structural characterization of the metal binding site in the cysteine-rich region of HIV-1 Tat protein.

@article{Huang1996StructuralCO,
  title={Structural characterization of the metal binding site in the cysteine-rich region of HIV-1 Tat protein.},
  author={H W Huang and K T Wang},
  journal={Biochemical and biophysical research communications},
  year={1996},
  volume={227 2},
  pages={615-21}
}
Human immunodeficiency virus type-1 Tat protein transactivates the gene expression of retrovirus. The unique cysteine-rich region of Tat protein is biologically essential. This study characterizes the structural features of a synthetic Tat21-38 peptide covering the cysteine-rich region with Zn binding property. UV titrations confirm Tat peptide binds with two Zn2+ cations maximally per monomer, as previously reported(1). Only monomer is observed from the electrospray mass spectrum… CONTINUE READING