Structural characterization of a novel full-length transcript promoter from Horseradish Latent Virus (HRLV) and its transcriptional regulation by multiple stress responsive transcription factors

  title={Structural characterization of a novel full-length transcript promoter from Horseradish Latent Virus (HRLV) and its transcriptional regulation by multiple stress responsive transcription factors},
  author={Ahamed Khan and Ankita Shrestha and Kashyap Bhuyan and Indu Bhushan Maiti and Nrisingha Dey},
  journal={Plant Molecular Biology},
Key messageThe promoter fragment described in this study can be employed for strong transgene expression under both biotic and abiotic stress conditions. [] Key Result The activity of full length transcript (Flt-) promoter from HRLV (− 677 to + 283) was investigated in both transient and transgenic assays where we identified H12 (− 427 to + 73) as the highest expressing fragment having ~ 2.5-fold stronger activity than the CaMV35S promoter.
Biochemical and Molecular Characterization of Novel Pararetroviral Promoters in Plants.
This chapter describes protocols used to determine the transgene integration and expression in transgenic plant systems and discusses the fusion reporter assays that can determine the promoter activity and DNA-protein interaction studies that aid in the evaluation of its transcriptional regulation.
Identification of Novel Pararetroviral Promoters for Designing Efficient Plant Gene Expression Systems.
This chapter will mainly discuss important protocols associated with identifying novel/unique pararetroviral promoters that have optimal lengths with appropriate activities for developing efficient plant gene expression systems.
Development of efficient synthetic promoters derived from pararetrovirus suitable for translational research.
The results had demonstrated that the developed promoter constructs could be used for translational research in dicot, monocot plants and bacterial systems for efficient gene expression, and they hold strong prominence in both transgenic research and biotech industries.
Synthetic Promoters from Strawberry Vein Banding Virus (SVBV) and Dahlia Mosaic Virus (DaMV)
Two intra-molecularly shuffled promoters, namely S100 and D100, have potential to become efficient candidates for plant metabolic engineering and molecular pharming and showed 1.8 and 2.2 times stronger activities than the CaMV35S promoter.
Identification of miRNA Targets by AtFT Overexpression in Tobacco
The heterologous expression of AtFT in tobacco may have potential in imparting abiotic stress tolerance in other plant species through plant biotechnology–based approaches.
Pararetroviruses: Plant Infecting dsDNA Viruses
The present review highlights the current taxonomy, genome structure, transmission, and diagnosis of pararetroviruses and how the “modular cassette” would help the modern biotechnology-based translational research.
YAP ISGylation increases its stability and promotes its positive regulation on PPP by stimulating 6PGL transcription
It is suggested that YAP ISGylation is critical for maintaining its stability and further activation of PPP, a new choice for hyperglycemia cancer treatment.


A Region Containing an as-1 Element of Dahlia Mosaic Virus (DaMV) Subgenomic Transcript Promoter Plays a Key Role in Green Tissue- and Root-Specific Expression in Plants
The binding of a tobacco transcription factor, TGA1a, that correlated with 2,4-dichlorophenylacetic acid (2,4D)-induced transcriptional activity of the DaMVSgt promoter may be useful for transgene containment applications.
Structure and promoter/leader deletion analysis of mirabilis mosaic virus (MMV) full-length transcript promoter in transgenic plants
A full-length transcript (FLt) promoter fragment was isolated from a genomic clone of mirabilis mosaic virus (MMV), a double-stranded DNA plant pararetrovirus belonging to the caulimovirus family and showed much greater activity than the CaMV 35S promoter.
Overexpression of the Tobacco Tsi1 Gene Encoding an EREBP/AP2–Type Transcription Factor Enhances Resistance against Pathogen Attack and Osmotic Stress in Tobacco
The results suggest that Tsi1 might be involved as a positive trans-acting factor in two separate signal transduction pathways under abiotic and biotic stress.
The 5′-region of Arabidopsis thaliana cor15a has cis-acting elements that confer cold-, drought- and ABA-regulated gene expression
Histochemical staining experiments and gene fusion experiments indicated that the 5′ region of cor15a between nucleotides −305 and +78 (relative to the start of transcription) contains a cis-acting element(s) that can impart cold-regulated gene expression.
Evolutionary conservation of transcriptional machinery between yeast and plants as shown by the efficient expression from the CaMV 35S promoter and 35S terminator
It is shown here that S. pombe initiates transcription at exactly the same start site as was reported for tobacco, and differential recognition of the 35S promoter is responsible for the different transcription rates.
NPR1 differentially interacts with members of the TGA/OBF family of transcription factors that bind an element of the PR-1 gene required for induction by salicylic acid.
The results directly link NPR1 to SA-induced PR-1 expression through members of the TGA family of transcription factors through the interaction of NPR1 with TGA2 and TGA3.
Gene VI of figwort mosaic virus (caulimovirus group) functions in posttranscriptional expression of genes on the full-length RNA transcript.
Experiments with various portions of the 5' leader of the large, full-length RNA of FMV showed that the coding region of gene VII is necessary for the transactivation event, and it appears that gene VI has a role in the posttranscriptional expression of the closely packed genes, which appear on the larger, full -length RNA transcript of this virus.
Activation of the CaMV as‐1 cis‐element by salicylic acid: differential DNA‐binding of a factor related to TGA1a.
Electrophoretic mobility shift assays demonstrated that the binding of a tobacco cellular factor, named SARP, correlates with the SA‐induced activation of transcription, suggesting the presence of an inhibitor that sequesters SARP.