Structural characterization of a K-antigen capsular polysaccharide essential for normal symbiotic infection in Rhizobium sp. NGR234: deletion of the rkpMNO locus prevents synthesis of 5,7-diacetamido-3,5,7,9-tetradeoxy-non-2-ulosonic acid.

@article{Qur2006StructuralCO,
  title={Structural characterization of a K-antigen capsular polysaccharide essential for normal symbiotic infection in Rhizobium sp. NGR234: deletion of the rkpMNO locus prevents synthesis of 5,7-diacetamido-3,5,7,9-tetradeoxy-non-2-ulosonic acid.},
  author={Antoine Le Qu{\'e}r{\'e} and William J. Deakin and C. Schmei\sser and Russell W Carlson and Wolfgang R. Streit and William J. Broughton and Lennart S Forsberg},
  journal={The Journal of biological chemistry},
  year={2006},
  volume={281 39},
  pages={28981-92}
}
Many early molecular events in symbiotic infection have been documented, although factors enabling Rhizobium to progress within the plant-derived infection thread and ultimately survive within the intracellular symbiosome compartment as mature nitrogen-fixing bacteroids are poorly understood. Rhizobial surface polysaccharides (SPS), including the capsular polysaccharides (K-antigens), exist in close proximity to plant-derived membranes throughout the infection process. SPSs are essential for… CONTINUE READING

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The complete structure of the K - antigen of strain NGR234 was established , and it consists of disaccharide repeating units of glucuronic and pseudaminic acid having the structure -->4)-beta - d - glucuronic acid-(1 - ->4)-beta-5,7-diacetamido-3,5,7,9-tetradeoxy - l - glycero - l - manno - nonulosonic acid-(2 - ->. Deletion of three genes located in the rkp-3 gene cluster , rkpM , rkpN , and part of rkpO , abolished pseudaminic acid synthesis , yielding a mutant in which the strain - specific K - antigen was totally absent : other surface glycoconjugates , including the lipopolysaccharides , exopolysaccharides , and flagellin glycoprotein appeared unaffected .
The complete structure of the K - antigen of strain NGR234 was established , and it consists of disaccharide repeating units of glucuronic and pseudaminic acid having the structure -->4)-beta - d - glucuronic acid-(1 - ->4)-beta-5,7-diacetamido-3,5,7,9-tetradeoxy - l - glycero - l - manno - nonulosonic acid-(2 - ->. Deletion of three genes located in the rkp-3 gene cluster , rkpM , rkpN , and part of rkpO , abolished pseudaminic acid synthesis , yielding a mutant in which the strain - specific K - antigen was totally absent : other surface glycoconjugates , including the lipopolysaccharides , exopolysaccharides , and flagellin glycoprotein appeared unaffected .
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