Structural changes accompanying enzymatic activation of human Hageman factor.

  title={Structural changes accompanying enzymatic activation of human Hageman factor.},
  author={Susan D. Revak and Charles G. Cochrane and Alex R. Johnston and Tony E. Hugli},
  journal={The Journal of clinical investigation},
  volume={54 3},
The structure of Hageman factor, isolated from human plasma, was analyzed before and after enzymatic activation. The purified molecule is a single polypeptide chain of 80,000 molecular weight (mol wt) sedimenting at 4.5S. An amino acid analysis has been performed. The concentration of Hageman factor in normal human plasma was found to be 29 mug/ml with variation between individuals ranging from 15 to 47 mug/ml. Treatment of the molecule with kallikrein, plasmin, or trypsin resulted in cleavage… CONTINUE READING


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