Structural change of threonine 89 upon photoisomerization in bacteriorhodopsin as revealed by polarized FTIR spectroscopy.

@article{Kandori1999StructuralCO,
  title={Structural change of threonine 89 upon photoisomerization in bacteriorhodopsin as revealed by polarized FTIR spectroscopy.},
  author={Hideki Kandori and N. Kinoshita and Yoichi Yamazaki and Akio Maeda and Yoshinori Shichida and Richard Needleman and Janos K. Lanyi and M Bizounok and Judith Herzfeld and Jan Raap and Johan Lugtenburg},
  journal={Biochemistry},
  year={1999},
  volume={38 30},
  pages={9676-83}
}
The all-trans to 13-cis photoisomerization of the retinal chromophore of bacteriorhodopsin occurs selectively, efficiently, and on an ultrafast time scale. The reaction is facilitated by the surrounding protein matrix which undergoes further structural changes during the proton-transporting reaction cycle. Low-temperature polarized Fourier transform infrared difference spectra between bacteriorhodopsin and the K intermediate provide the possibility to investigate such structural changes, by… CONTINUE READING