Structural biology of transmembrane domains: efficient production and characterization of transmembrane peptides by NMR.

@article{Hu2007StructuralBO,
  title={Structural biology of transmembrane domains: efficient production and characterization of transmembrane peptides by NMR.},
  author={Jian Hu and Huajun Qin and Conggang Li and Mukesh Sharma and Timothy A. Cross and Fei Gao},
  journal={Protein science : a publication of the Protein Society},
  year={2007},
  volume={16 10},
  pages={
          2153-65
        }
}
Structural characterization of transmembrane peptides (TMPs) is justified because transmembrane domains of membrane proteins appear to often function independently of the rest of the protein. However, the challenge in obtaining milligrams of isotopically labeled TMPs to study these highly hydrophobic peptides by nuclear magnetic resonance (NMR) is significant. In the present work, a protocol is developed to produce, isotopically label, and purify TMPs in high yield as well as to initially… CONTINUE READING

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