Structural biology: Breaking the diffraction barrier

Abstract

Short single-stranded DNA donors that asymmetrically span the Cas9 cut site show high efficiency in homology-directed editing. Among the many attractive features of the CRISPR–Cas9 system is its ability to replace a target sequence with a donor of choice. The efficiency of this homologydirected repair (HDR) varies widely depending on the cell type and… (More)
DOI: 10.1038/nmeth.3825

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