Structural basis of initial RNA polymerase II transcription

  title={Structural basis of initial RNA polymerase II transcription},
  author={Alan C M Cheung and Sarah Sainsbury and Patrick Cramer},
  booktitle={The EMBO journal},
During transcription initiation by RNA polymerase (Pol) II, a transient open promoter complex (OC) is converted to an initially transcribing complex (ITC) containing short RNAs, and to a stable elongation complex (EC). We report structures of a Pol II-DNA complex mimicking part of the OC, and of complexes representing minimal ITCs with 2, 4, 5, 6, and 7 nucleotide (nt) RNAs, with and without a non-hydrolyzable nucleoside triphosphate (NTP) in the insertion site +1. The partial OC structure… CONTINUE READING
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