Structural basis of gene regulation by the tetracycline inducible Tet repressor–operator system

@article{Orth2000StructuralBO,
  title={Structural basis of gene regulation by the tetracycline inducible Tet repressor–operator system},
  author={Peter Orth and D. Schnappinger and W. Hillen and W. Saenger and W. Hinrichs},
  journal={Nature Structural Biology},
  year={2000},
  volume={7},
  pages={215-219}
}
  • Peter Orth, D. Schnappinger, +2 authors W. Hinrichs
  • Published 2000
  • Medicine, Biology
  • Nature Structural Biology
  • The tetracycline repressor (TetR) regulates the most abundant resistance mechanism against the antibiotic tetracycline in gram-negative bacteria. The TetR protein and its mutants are commonly used as control elements to regulate gene expression in higher eukaryotes. We present the crystal structure of the TetR homodimer in complex with its palindromic DNA operator at 2.5 Å resolution. Comparison to the structure of TetR in complex with the inducer tetracycline-Mg2+ allows the mechanism of… CONTINUE READING

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    The role of ligand induced stabilization in the allosteric mechanism of tetracycline repressor.

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