Structural basis of enhanced photoconversion yield in green fluorescent protein-like protein Dendra2.

@article{Adam2009StructuralBO,
  title={Structural basis of enhanced photoconversion yield in green fluorescent protein-like protein Dendra2.},
  author={Virgile Adam and Karin Nienhaus and Dominique Bourgeois and Gerd Ulrich Nienhaus},
  journal={Biochemistry},
  year={2009},
  volume={48 22},
  pages={
          4905-15
        }
}
Dendra2 is an engineered, monomeric GFP-like protein that belongs to a subclass of fluorescent proteins undergoing irreversible photoconversion from a green- to a red-emitting state upon exposure to purple-blue light. This photoinduced process occurs only in the neutral state of the chromophore and is known to result from backbone cleavage accompanied by an extension of the delocalized pi-electron system. We have measured the X-ray structure of the green species of Dendra2 and performed a… Expand
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