Structural basis for unfolding pathway-dependent stability of proteins: vectorial unfolding versus global unfolding.

@article{Yagawa2010StructuralBF,
  title={Structural basis for unfolding pathway-dependent stability of proteins: vectorial unfolding versus global unfolding.},
  author={Keisuke Yagawa and Koji Yamano and Takaomi Oguro and Masahiro Maeda and Takehiro Sato and Takaki Momose and Shin Kawano and Toshiya Endo},
  journal={Protein science : a publication of the Protein Society},
  year={2010},
  volume={19 4},
  pages={
          693-702
        }
}
Point mutations in proteins can have different effects on protein stability depending on the mechanism of unfolding. In the most interesting case of I27, the Ig-like module of the muscle protein titin, one point mutation (Y9P) yields opposite effects on protein stability during denaturant-induced "global unfolding" versus "vectorial unfolding" by mechanical pulling force or cellular unfolding systems. Here, we assessed the reason for the different effects of the Y9P mutation of I27 on the… CONTINUE READING