Structural basis for the recognition of guide RNA and target DNA heteroduplex by Argonaute

@inproceedings{Miyoshi2016StructuralBF,
  title={Structural basis for the recognition of guide RNA and target DNA heteroduplex by Argonaute},
  author={Tomohiro Miyoshi and Kosuke Ito and Ryo Murakami and Toshio Uchiumi},
  booktitle={Nature communications},
  year={2016}
}
Argonaute proteins are key players in the gene silencing mechanisms mediated by small nucleic acids in all domains of life from bacteria to eukaryotes. However, little is known about the Argonaute protein that recognizes guide RNA/target DNA. Here, we determine the 2 Å crystal structure of Rhodobacter sphaeroides Argonaute (RsAgo) in a complex with 18-nucleotide guide RNA and its complementary target DNA. The heteroduplex maintains Watson-Crick base-pairing even in the 3'-region of the guide… CONTINUE READING
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