Structural basis for nick recognition by a minimal pluripotent DNA ligase

  title={Structural basis for nick recognition by a minimal pluripotent DNA ligase},
  author={Pravin A. Nair and Jayakrishnan Nandakumar and Paul Smith and Mark Odell and Christopher D Lima and Stewart Shuman},
  journal={Nature Structural &Molecular Biology},
Chlorella virus DNA ligase, the smallest eukaryotic ligase known, has pluripotent biological activity and an intrinsic nick-sensing function, despite having none of the accessory domains found in cellular ligases. A 2.3-Å crystal structure of the Chlorella virus ligase-AMP intermediate bound to duplex DNA containing a 3′-OH–5′-PO4 nick reveals a new mode of DNA envelopment, in which a short surface loop emanating from the OB domain forms a β-hairpin 'latch' that inserts into the DNA major… CONTINUE READING

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structure of E

  • J. Nandakumar, P. A. Nair, Shuman, S. Last stop on the road to repair
  • coli DNA ligase bound to nicked DNA-adenylate…
  • 2007

Crystal structure and nonhomologous end joining function of the ligase domain of Mycobacterium DNA ligase D

  • D Akey
  • J. Biol
  • 2006

Identification of a novel motif in DNA ligases exemplified by DNA ligase IV

  • C Marchetti
  • DNA Repair (Amst.)
  • 2006

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