Structural basis for high-affinity binding of LEDGF PWWP to mononucleosomes

  title={Structural basis for high-affinity binding of LEDGF PWWP to mononucleosomes},
  author={Jocelyn O. Eidahl and Brandon L. Crowe and Justin A. North and Christopher J. McKee and Nikoloz Shkriabai and Lei Feng and Matthew R. Plumb and Robert L.J. Graham and Robert J. Gorelick and Sonja Hess and Michael Guy Poirier and Mark P. Foster and Mamuka Kvaratskhelia},
  booktitle={Nucleic acids research},
Lens epithelium-derived growth factor (LEDGF/p75) tethers lentiviral preintegration complexes (PICs) to chromatin and is essential for effective HIV-1 replication. LEDGF/p75 interactions with lentiviral integrases are well characterized, but the structural basis for how LEDGF/p75 engages chromatin is unknown. We demonstrate that cellular LEDGF/p75 is tightly bound to mononucleosomes (MNs). Our proteomic experiments indicate that this interaction is direct and not mediated by other cellular… CONTINUE READING


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