Structural basis for gate-DNA recognition and bending by type IIA topoisomerases

  title={Structural basis for gate-DNA recognition and bending by type IIA topoisomerases},
  author={Ken C Dong and James M. Berger},
Type II topoisomerases disentangle DNA to facilitate chromosome segregation, and represent a major class of therapeutic targets. Although these enzymes have been studied extensively, a molecular understanding of DNA binding has been lacking. Here we present the structure of a complex between the DNA-binding and cleavage core of Saccharomyces cerevisiae Topo II (also known as Top2) and a gate-DNA segment. The structure reveals that the enzyme enforces a 150° DNA bend through a mechanism similar… 
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