Structural basis for cooperative DNA binding by CAP and lac repressor.

  title={Structural basis for cooperative DNA binding by CAP and lac repressor.},
  author={Alexander Balaeff and Lakshminarayanan Mahadevan and Klaus Schulten},
  volume={12 1},
Catabolite gene activator protein (CAP) and lac repressor (LR) are celebrated transcription-regulating proteins that bind to DNA cooperatively forming a ternary complex with the promoter loop. Here we present a multiscale model of the ternary complex derived from crystal structures of the proteins and a continuous structure of the DNA loop built using the theory of elasticity. We predict that the loop is underwound in the binary complex with the LR, whereas in the ternary complex with the LR… CONTINUE READING


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